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Preferably, just one would want to engineer a DNA ligase mutant able to binding an adenosine analog that's Usually excluded in the Energetic site with the wild-style ligase. Changing the wild-form ligase Together with the analog-delicate ligase in an organism that has several ligase isozymes could make it possible for selective and quick inhibition of strand sealing, without having depleting the ligase protein and devoid of impacting the numerous other proteins that connect with DNA ligases in mammalian cells (2, three).
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